A signaling pathway contributing to platelet storage lesion development: targeting PI3-kinase-dependent Rap1 activation slows storage-induced platelet deterioration
نویسندگان
چکیده
منابع مشابه
RASA3 is a critical inhibitor of RAP1-dependent platelet activation.
The small GTPase RAP1 is critical for platelet activation and thrombus formation. RAP1 activity in platelets is controlled by the GEF CalDAG-GEFI and an unknown regulator that operates downstream of the adenosine diphosphate (ADP) receptor, P2Y12, a target of antithrombotic therapy. Here, we provide evidence that the GAP, RASA3, inhibits platelet activation and provides a link between P2Y12 and...
متن کاملA mitogen-activated protein kinase-dependent signaling pathway in the activation of platelet integrin alpha IIbbeta3.
We have recently shown that the platelet integrin alpha(IIb)beta(3) is activated by von Willebrand factor (vWF) binding to its platelet receptor, glycoprotein Ib-IX (GPIb-IX), via the protein kinase G (PKG) signaling pathway. Here we show that GPIb-IX-mediated activation of integrin alpha(IIb)beta(3) is inhibited by dominant negative mutants of Raf-1 and MEK1 in a reconstituted integrin activat...
متن کاملAn Overview on Platelet-derived Microparticles in Platelet Concentrates: blood collection, method preparation and storage
Preparations of platelet concentrates (PCs) that are stored under blood bank conditions and used for transfusion purposes, appear to be enriched in platelet derived-microparticles (PMPs) with high coagulant activity that may change platelet efficacy and safety issues. High shear stress could cause shedding of PMPs from the platelet plasma membrane, platelet aggregation, and activation of the co...
متن کاملShedding New Light on the Platelet Storage Lesion.
The lifespan of circulating endogenous platelets is determined by a combination of random platelet removal and a nonrandom aging mechanism after which senescent platelets are removed by the reticuloendothelial system. At least a portion of platelet senescence is regulated by an intrinsic apoptotic pathway that acts as an internal timer. Platelets contain members of the antiapoptotic Bcl-2 famil...
متن کاملEnhanced shear-induced platelet aggregation due to low-temperature storage.
BACKGROUND Refrigeration of platelets (PLTs) offers an attractive alternative to the currently practiced storage at room temperature since it may mitigate problems associated with bacterial contamination and extend storage lifetime. Refrigeration causes a number of biophysical and biochemical changes in PLTs and decreases PLT circulation time in vivo. However, the effect of refrigeration on PLT...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Transfusion
سال: 2009
ISSN: 0041-1132
DOI: 10.1111/j.1537-2995.2009.02224.x